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5-[3-(生物素酰氨基己酰)-胺基]-丙烯基-2’-去氧-5’-三磷酸脲嘧啶核苷(VI)和5-[3-(生物素酰氨基己酰)-胺基]-丙烯基-2’-去氧-5’-三磷酸胞嘧啶核苷(Ⅶ)从d-CTP原料经四步反应合成。用(Ⅵ)和(Ⅶ)标记的生物素DNA探针已用于检测乙肝病毒,c-Myc基因和人类多瘤病毒等。
Synthesis of 5- [3- (biotinamidocaproyl) -amino] -propenyl-2’-deoxy-5’-triphosphate uridine (VI) and 5- [3- (biotinamido Hexanoyl) -amino] -propenyl-2’-deoxy-5’-triphosphate (VII) was synthesized from the d-CTP starting material in four steps. The biotin DNA probes labeled with (Ⅵ) and (Ⅶ) have been used to detect hepatitis B virus, c-Myc gene and human polyoma virus.