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本工作用显示cAMP的免疫荧光细胞化学方法观察了小鼠艾氏腹水癌细胞内cAMP的分布,并发现经外源性cAMP及氨茶碱处理后,有使小鼠艾氏腹水癌细胞内cAMP荧光增强的效应。cAMP主要分布在胞质内。实验组动物从接种癌细胞的次日起,每日从腹腔注射2毫克的cAMP和1毫克的氨茶碱,发现在接种后第9天,大多数癌细胞胞质内的cAMP荧光增强,以核周区胞质内的荧光最显著。进一步证实了我室以前用生化测定癌细胞内cAMP含量的结果。本文并对显示cAMP的免疫荧光细胞化学方法进行了讨论,用cAMP荧光染色反应的特异性鉴定证明了方法的可靠性。
In this work, cAMP immunofluorescence cytochemistry was used to observe the distribution of cAMP in Ehrlich ascites carcinoma cells. It was found that after treatment with exogenous cAMP and aminophylline, the mice were infected with E. coli. Fluorescence enhancement effect. cAMP is mainly distributed in the cytoplasm. In the experimental group, 2 mg of cAMP and 1 mg of aminophylline were injected intraperitoneally from the day after the cancer cells were inoculated. It was found that on the 9th day after inoculation, the fluorescence of cAMP in the cytoplasm of most cancer cells was enhanced. The fluorescence in the cytoplasm of the perinuclear zone is the most significant. It further confirmed the results of biochemical assay of cAMP content in cancer cells in our hospital. In this paper, the immunofluorescence cytochemistry method for displaying cAMP was discussed. The specific identification of the cAMP fluorescence staining reaction demonstrated the reliability of the method.