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目的:探讨多索茶碱对支气管哮喘(简称哮喘)大鼠肺部炎症的影响,以及对哮喘发病时白细胞介素-5在肺部沉积的影响。方法:(1)免疫组织化学法检测白细胞介素-5在肺部沉积的情况;(2)肺组织学检查;(3)酶联免疫吸附实验检测外周血总IgE水平。结果:哮喘组支气管肺泡灌洗液中及血清中IgE的水平明显高于对照组(P<0.05);白细胞介素-5在肺部的沉积也明显多于对照组;而多索茶碱干预组IgE水平明显低于哮喘组(P<0.05),肺组织病变减轻,白细胞介素-5的沉积也明显减少(P<0.05)。结论:多索茶碱能减少白细胞介素-5在肺部的沉积,从而减少气道的高反应性。
Objective: To investigate the effects of doxofylline on lung inflammation in rats with bronchial asthma (asthma) and its effect on the deposition of interleukin-5 in lung during the onset of asthma. Methods: (1) Immunohistochemistry was used to detect the deposition of interleukin-5 in the lungs; (2) Lung histology; (3) Total IgE levels in peripheral blood were detected by enzyme-linked immunosorbent assay. Results: The level of IgE in bronchoalveolar lavage fluid and serum in asthmatic group was significantly higher than that in control group (P <0.05); the deposition of interleukin-5 in lung was also significantly more than that in control group; while Doxofylline intervention The level of IgE in asthma group was significantly lower than that in asthma group (P <0.05). The lung lesions were alleviated and the deposition of interleukin-5 was also significantly decreased (P <0.05). Conclusion: Doxofylline can reduce the deposition of interleukin-5 in the lungs and thus reduce airway hyperresponsiveness.