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目的了解南山区副溶血性弧菌血清群分布及流行趋势。方法依据GB/T4789.7-2003方法,对食物中毒病人肛拭样本及日常临测的食品和水产品样品进行副溶血性弧菌分离、培养及血清学鉴定,数据进行统计及分析。结果共分离到分离副溶血性弧菌599株,分属11个血清群,主要为O3、O1、O4、O5血清群,共占78.62%。其中食物中毒病人分离454株,主要血清群为O3占61.67%(280/454),O1占12.33%(56/454),O4占9.69%(44/454);食品和水产品样品分离145株,主要血清群为O3占24.83%(36/145),O1占12.41%(18/145),O4占10.34%(15/145),O5占8.97%(13/145)。结论食物中毒病人肛拭样本分离的副溶血性弧菌与食品和水产品样品分离的副溶血性弧菌血清群都以O3,O1,O4为主,食物中毒病人分离株血清分群缺少O7,O9,O10三个血清群,但食品和水产品样品分离株血清群O1～O11都有,更具血清分群多样化特点。
Objective To understand the distribution and epidemic trend of serogroups of Vibrio parahaemolyticus in Nanshan District. Methods According to the method of GB / T4789.7-2003, samples of anal swab of food poisoning patients and samples of food and aquatic products which were routinely tested were isolated, cultured and serologically confirmed. The data were statistically analyzed. Results A total of 599 isolates of Vibrio parahaemolyticus were isolated, belonging to 11 serogroups, mainly O3, O1, O4 and O5 serogroups, accounting for 78.62%. Among them, 454 strains were isolated from food poisoning patients, the main serogroups were O61 accounting for 61.67% (280/454), O1 accounting for 12.33% (56/454) and O4 accounting for 9.69% (44/454) The major serogroups were O3 accounting for 24.83% (36/145), O1 accounting for 12.41% (18/145), O4 accounting for 10.34% (15/145) and O5 accounting for 8.97% (13/145). Conclusion The Vibrio parahaemolyticus isolated from the anal swab samples of food poisoning patients and the Vibrio parahaemolyticus serogroups isolated from food and aquatic samples are mainly O3, O1 and O4, and the isolates from food poisoning patients lack O7, O9 , O10 three serogroups, but the food and aquatic product isolates serogroups O1 ~ O11 have more serogroup diversification characteristics.