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为探讨紫贻贝体内重金属Cd残留的脱除方法,采用海水中添加制备的蛋白水解肽-Fe2+配合物(TPH-Fe2+)进行暂养,检测贻贝不同组织器官中Cd含量变化情况。结果显示,贻贝不同器官中Cd富集量,依次为内脏团>鳃>后闭壳肌>外套膜>前闭壳肌,且在0~10 d自然净化过程中,无显著性下降趋势(P>0.05)。添加10 mg/L TPH-Fe2+净化处理,对贻贝内脏团及鳃中Cd含量无显著性影响;添加15~25 mg/L TPH-Fe2+净化处理贻贝4 d后,内脏团、鳃、前后闭壳肌及外套膜中Cd含量均有不同程度下降(P<0.05);20 mg/L TPH-Fe2+净化10 d后,贻贝不同器官中Cd脱除率表现为鳃>内脏团>外套膜>前后闭壳肌,最高脱除率分别为41.00%、37.43%、29.81%和29.01%,与25 mg/L TPH-Fe2+净化处理组无显著性差异(P>0.05)。研究表明,蛋白水解肽-Fe2+配合物有效脱除贻贝体内Cd残留,可作为一种双壳贝类养殖饲料添加剂加以应用。
In order to investigate the method of removal of heavy metal Cd residues in mussels, the protein content of Cd in different tissues and organs of mussels was measured by holding them in seawater with TPH-Fe2 +. The results showed that Cd enrichment in different organs of mussels followed by visceral mass> gill> posterior closed vessel> mantle> anterior closed shell muscle, and there was no significant decrease in Cd during the natural purification from 0 to 10 days P> 0.05). Feeding with 10 mg / L TPH-Fe2 + had no significant effect on Cd content in visceral mass and gills of mussels. After adding 4 mg / L TPH-Fe2 + for 15 days, the visceral mass, gill, (P <0.05). After 20 mg / L TPH-Fe2 + was purged for 10 days, the Cd removal rate in different organs of mussels showed gill> visceral mass> mantle (P <0.05). The highest removal rates of adductor muscle were 41.00%, 37.43%, 29.81% and 29.01% respectively, which were not significantly different from those of 25 mg / L TPH-Fe2 + group. Studies have shown that proteolytic peptide-Fe2 + complexes can effectively remove Cd residues in mussels and can be used as a bivalve mollusc feed additive.