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目的:采用高效液相色谱法测定五加生化胶囊中阿魏酸的含量。方法:色谱柱:Kromasil C_(18)柱(250mm×4.6mm, 5μm),流动相:乙腈-0.1%磷酸水溶液(15:85);流速:0.8 ml·min~(-1);检测波长:323nm;柱温:30℃。结果:阿魏酸在2.4～19.2μg·ml~(-1)浓度范围与峰面积具有良好的线性关系(r=0.999 9);平均加样回收率为99.16%,RSD为0.98%(n=5)。结论:本方法简便、快速准确,可作为该制剂阿魏酸的含量测定。
Objective: To determine the content of ferulic acid in Wujia Biochemical Capsule by high performance liquid chromatography. Methods: The chromatographic column was Kromasil C 18 column (250 mm × 4.6 mm, 5 μm). The mobile phase consisted of acetonitrile-0.1% phosphoric acid solution (15:85), the flow rate was 0.8 ml · min -1, 323 nm; column temperature: 30 ° C. Results: Ferulic acid showed good linearity (r = 0.999 9) in the range of 2.4 ~ 19.2μg · ml ~ (-1) with an average recovery of 99.16% and a RSD of 0.98% (n = 5). Conclusion: The method is simple, rapid and accurate and can be used as the determination of the content of ferulic acid.