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目的 建立测定川东獐牙菜中獐牙菜苦苷和去甲当药苷含量的高效液相色谱方法。方法 HYPERSILBDSC18(4.6 mm×200 mm,5μm)色谱柱,流动相为乙腈-磷酸二氢钠(0.03 mol·L-1,pH=3.0),梯度洗脱(ACN%;0 min 5％,20 min 15％),流速为0.8 mL·min-1,检测波长为250 nm,柱温为25℃。结果 獐牙菜苦苷和去甲当药苷分别在1.528-11.466μg,85.4-854 ng范围内线性良好,相关系数r分别为0.99992、0.99988, 平均回收率分别为99.1％和101.8％,RSD均<1％(n=5)。结论 本方法操作简便,分离效果好,灵敏度高,重 现性好,为控制川东獐牙菜质量提供参考。
Objective To establish a high performance liquid chromatographic method for the determination of the contents of campestrin and norgamidin in Swertia chuanxiong. Method HYPERSIL BDSC18 (4.6 mm×200 mm, 5 μm) column with mobile phase of acetonitrile-sodium dihydrogen phosphate (0.03 mol·L-1, pH=3.0), gradient elution (ACN%; 0 min 5%, 20 min 15%), flow rate 0.8 mL·min-1, detection wavelength 250 nm, column temperature 25°C. RESULTS: The linearity of the swertiamarin and nordangidine in the ranges of 1.528-11.466μg and 85.4-854 ng were good, and the correlation coefficients r were 0.99992 and 0.99988, respectively. The average recoveries were 99.1% and 101.8%, respectively. <1% (n=5). Conclusion This method is easy to operate, has good separation effect, high sensitivity and good reproducibility. It provides a reference for controlling the quality of Swertia chuanxiong.