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近年来,世界各国肺癌的发病率和死亡率急剧上升。肺癌按细胞分化程度和形态特征分为鳞癌、小细胞癌、大细胞未分化癌、腺癌、肺泡癌,及早期诊断并分型对临床治疗有很大帮助。因此,我们对152例肺癌病人血清中的非小细胞肺癌标志物（CYFRA21-1）和神经元特异性烯醇化酶（NSE）进行了检测,并初步探讨其对肺癌的诊断价值。 材料和方法 一、对象: （一）肺癌组:152例（男96,女56）,年龄40～83岁,平均62岁。均为本院住院病人,并经临床、影像、实验室和病理检查证实。 （二）正常对照组:71例（男41,女30）,年龄20～73岁,平均43岁。均为本院健康体检合格者,无心、肝、肺、肾等重要脏器疾病。 二、方法: （一）清晨空腹抽取肘静脉血,及时分离血清,-20℃冻存待检。 （二）CYFRA 21-1放免试剂盒由法国CIS公司提供;NSE酶联免疫试剂盒由北京军事医学院放射医学研究所提供,均按说明书操作。γ-计数仪为芬兰产全自动WIA-γ计数仪,酶标仪为奥地利产Clinbio 128型全自动酶标仪。 结果 我们以CYFRA 21-1>3.3U>ml,NSE>14ng/ml作为阳性界值,测定结果见表1。两项联合检测结果分析见表2。
In recent years, the incidence and death rate of lung cancer have risen sharply in various countries around the world. By lung cancer cell differentiation and morphological characteristics into squamous cell carcinoma, small cell carcinoma, large cell undifferentiated carcinoma, adenocarcinoma, alveolar carcinoma, and early diagnosis and classification of great help for clinical treatment. Therefore, we have 152 cases of lung cancer patients serum in non-small cell lung cancer markers (CYFRA21-1) and neuron-specific enolase (NSE) were detected, and to explore its diagnostic value of lung cancer. Materials and Methods 1. Object: (1) Lung cancer group: 152 cases (male 96, female 56), aged 40-83 years old, average 62 years old. All patients were hospitalized in our hospital and confirmed by clinical, imaging, laboratory and pathological examinations. (B) normal control group: 71 cases (41 males and 30 females), aged 20 to 73 years, an average of 43 years old. All of them are qualified for physical examination in this hospital, and they have no heart, liver, lung, kidney and other important organ diseases. Second, methods: (A) fasting in the early morning to take elbow venous blood, timely separation of serum, -20 °C frozen deposit pending inspection. (2) The CYFRA 21-1 radioimmunoassay kit is provided by the French CIS company; the NSE enzyme-linked immunoassay kit is provided by the Institute of Radiological Medicine, Beijing Military Medical College, and is operated according to the instructions. The gamma-counter is a fully automatic WIA-gamma counter produced in Finland, and the microplate reader is an Austrian Clinbio 128 automatic microplate reader. Results We used CYFRA 21-1>3.3 U>ml and NSE>14 ng/ml as positive boundary values. The results are shown in Table 1. The results of the two joint test results are shown in Table 2.