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目的观察蛇床子素对大鼠动脉粥样硬化形成的影响。方法健康雄性SD大鼠随机分为6组,即正常对照组、模型组、蛇床子素大剂量组(40 mg/kg)、蛇床子素中剂量组(20 mg/kg)、蛇床子素小剂量组(10 mg/kg)和罗格列酮组(4 mg/kg)。在给药的同时采用高脂乳剂灌胃加一次腹腔注射维生素D3法复制动脉粥样硬化大鼠模型,连续给药6周后,检测大鼠血清总胆固醇(TC)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)、肿瘤坏死因子-α(TNF-α)含量以及肝中TC、甘油三酯(TG)的含量。用光镜检查胸主动脉的病理变化。结果蛇床子素能显著降低动脉粥样硬化大鼠血清中TC和LDL-C含量以及LDL-C/HDL-C比值(均P<0.01),蛇床子素也能同时显著降低血清中TNF-α的含量(P<0.01),蛇床子素40 mg/kg能显著降低肝组织中TC的含量(P<0.01)。光镜检测结果显示蛇床子素能改善大鼠早期动脉粥样硬化的形态学改变。结论蛇床子素可通过它的调脂和抗炎作用抑制大鼠动脉粥样硬化的形成。
Objective To observe the effects of osthole on the formation of atherosclerosis in rats. Methods Healthy male Sprague-Dawley rats were randomly divided into 6 groups: normal control group, model group, high dose of Osthol (40 mg / kg), middle dose of Osthol (20 mg / kg) Dose group (10 mg / kg) and rosiglitazone group (4 mg / kg). At the same time of administration, the model of atherosclerosis was replicated by intragastric administration of high-fat emulsion plus intraperitoneal injection of vitamin D3. After continuous administration for 6 weeks, serum total cholesterol (TC), high density lipoprotein cholesterol HDL-C), low density lipoprotein cholesterol (LDL-C), tumor necrosis factor-α (TNF-α) and liver TC, triglyceride (TG) Using light microscopy examination of the pathological changes of the thoracic aorta. Results Osthole could significantly decrease the content of TC and LDL-C and the ratio of LDL-C / HDL-C in atherosclerotic rats (all P <0.01). Osthole could also significantly decrease the levels of TNF-α (P <0.01). Osthol 40 mg / kg significantly reduced the content of TC in liver tissue (P <0.01). Light microscopy results showed that osthole can improve the morphology of early atherosclerosis in rats. Conclusion Osthole can inhibit the formation of atherosclerosis in rats through its lipid-lowering and anti-inflammatory effects.