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目的:研究AKR1C1(Aldo-keto reductase family 1 member C1)在胃癌中的表达,并初步探讨其在胃癌发生和发展中的作用。方法:采用胃癌组织芯片和免疫组化分析方法,检测60例胃癌患者癌组织及癌旁正常组织AKR1C1的表达;实时荧光定量PCR和蛋白免疫印迹western blot检测胃癌细胞株SGC-7901 AKR1C1的表达;在胃癌细胞株SGC-7901中转染沉默AKR1C1的sh RNA质粒及空质粒,MTT比色法检测各实验组细胞增殖。结果:组织芯片和免疫组化结果显示,与正常组织相比,胃癌组织中AKR1C1呈高表达;实时荧光定量PCR和western blot观察可以发现胃癌细胞株SGC-7901高表达AKR1C1;MTT比色法检测发现,转染沉默AKR1C1的sh RNA质粒组与空质粒对照组相比,SGC-7901细胞的增殖受到明显抑制,差异有统计学意义(P<0.05)。结论:AKR1C1与癌细胞的增殖有关,可能是其参与或间接参与了癌细胞的生长周期,为胃癌的发生及细胞增值提供了新的研究思路和方向。
OBJECTIVE: To study the expression of AKR1C1 (Aldo-keto reductase family 1 member C1) in gastric cancer and to investigate the role of AKR1C1 in the development and progression of gastric cancer. Methods: Gastric cancer tissues and immunohistochemical methods were used to detect the expression of AKR1C1 in 60 cases of gastric cancer and adjacent normal tissues. The expression of AKR1C1 in gastric cancer cell line SGC-7901 was detected by real-time fluorescence quantitative PCR and Western blotting. ShRNA plasmid and empty plasmid of silenced AKR1C1 were transfected into gastric cancer cell line SGC-7901. Cell proliferation was detected by MTT assay. Results: Tissue microarray and immunohistochemistry results showed that AKR1C1 was highly expressed in gastric cancer tissues compared with normal tissues. AKR1C1 was highly expressed in gastric cancer cell line SGC-7901 by real-time fluorescence quantitative PCR and western blot. MTT colorimetric assay The results showed that the proliferation of SGC-7901 cells was significantly inhibited compared with the empty plasmid control group transfected with silenced AKR1C1 sh RNA plasmid group (P <0.05). CONCLUSION: AKR1C1 is related to the proliferation of cancer cells, which may be involved in or indirectly involved in the growth cycle of cancer cells, providing new research ideas and directions for the occurrence of gastric cancer and cell proliferation.