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目的:研究白花丹地上部分的抗氧化活性成分。方法:利用硅胶柱层析、中压柱层析(RP C18色谱柱)、制备型HPLC(RP C18色谱柱)等色谱技术对白花丹地上部分乙醇提取部位的化学成分进行分离纯化。通过ESIMS、UV、1H-NMR、13C-NMR等波谱学手段结合文献调研对所分离得到的化学成分进行结构测定。采用清除ABTS+自由基法对所得到的单体化合物进行抗氧化活性评价。结果:从白花丹地上部分中共分离鉴定了11个化合物,分别为:反-异柿萘醇酮-4-O-β-D-吡喃葡萄糖苷(1)、tachioside(2)、2,6-二甲氧基-对苯二酚-1-O-β-D-吡喃葡萄糖苷(3)、3-(β-D-吡喃葡萄糖基)-4-甲氧基苯甲酸(4)、3’-O-β-D-吡喃葡萄糖基-白花丹酸(5)、3’-O-β-D-吡喃葡萄糖基-白花丹酸甲酯(6)、白花丹酸(7)、plumbagine A(8)、plumbagine C(9)、丁香酸-4-O-β-D-吡喃葡萄糖苷(10)、2-甲基-5-羟基色原酮(11)。抗氧化活性评价显示化合物2、3、5具有较为显著的ABTS+自由基清除能力。结论:其中,化合物1~4、10、11为首次从该植物中分离得到;化合物2、3、5具有显著的抗氧化活性。
Objective: To study the antioxidant activity of the aerial part Methods: The chemical constituents from the ethanol extract from the aerial part of Pseudosupuum cameata were isolated and purified by silica gel column chromatography, medium pressure column chromatography (RP C18) and preparative HPLC (RP C18 column). The structures of the isolated chemical constituents were determined by spectroscopic methods such as ESIMS, UV, 1H-NMR and 13C-NMR. The ABTS + radical scavenging method was used to evaluate the antioxidant activity of the obtained monomeric compounds. Results: Eleven compounds were isolated and identified from the aerial parts of Pseudostellaria baicalensis. They were tachioside (2), 2,6 (3), 3- (β-D-glucopyranosyl) -4-methoxybenzoic acid (4) , 3’-O-β-D-glucopyranosyl-leucovorin (5), methyl 3’-O-β-D-glucopyranosyl- ), Plumbagine A (8), plumbagine C (9), syringic acid-4-O-β-D-glucopyranoside (10) and 2-methyl-5-hydroxy chromogen (11). Antioxidant activity evaluation showed that compounds 2, 3 and 5 had more significant ABTS + free radical scavenging ability. Conclusion: Among them, compounds 1 ~ 4, 10 and 11 were isolated from this plant for the first time. Compounds 2, 3 and 5 have significant antioxidant activity.