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探讨小肠缺血预适应(IP)对缺血再灌注(I/R)损伤肠黏膜保护机理。通过用无创动脉夹夹闭肠系膜前动脉(SMA)30 m in,再松夹灌注60 m in以制造I/R模型;夹8 m in松10 m in反复3次以制造IP模型;采用改良AKP组织化学方法显示要观察测量的肠黏膜微血管,用方网格测试系统进行微血管体视学测量。与对照组相比,I/R组及IP+I/R组均可见血管腔闭塞等组织学变化,I/R组比IP+I/R组严重;I/R组和IP+I/R组肠黏膜固有层微血管的体积密度(Vv)、长度密度(Lv)、平均截面面积(-A)和平均周长(B-)均比对照组的小,但IP组明显比I/R组更大(均p<0.01)。结果表明小肠缺血预适应对缺血再灌注肠黏膜微血管损伤变化具有明显的保护作用,能改善局部微循环而减少组织损伤。
To investigate the protective mechanism of intestinal ischemia preconditioning (IP) on intestinal mucosal injury induced by ischemia / reperfusion (I / R) injury. The I / R model was made by clamping the SMA for 30 mins with a noninvasive arterial clamp and a 60 min perfusion with a loose clamp for 3 min to create an IP model. A modified AKP Histochemical methods showed that the gut microvessels to be measured were observed and the microvascular volume was measured with a square grid test system. Compared with the control group, histological changes such as vessel occlusion were observed in I / R group and IP + I / R group, and I / R group was more severe than IP + I / R group; The volume density (Vv), length density (Lv), average cross-sectional area (-A) and average circumference (B-) of the intestinal mucosa in the lamina propria were smaller than those in the control group Larger (all p <0.01). The results show that intestinal ischemia preconditioning has a significant protective effect on the changes of intestinal mucosal microvascular injury after ischemia-reperfusion, can improve local microcirculation and reduce tissue damage.