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分离与鉴定莱姆病螺旋体.方法：1996年在山东省费县塔山林场，对莱姆病螺旋体的主要媒介蜱进行调查，用布旗法共采集蜱900只，均为长角血蜱。结果：对50只进行中肠带螺旋体检查，带螺旋体率为12％（6／50）。从86组（850余只）长角血蜱中分离到2株螺旋体（TSH1、TSH3）。该螺旋体对H5332和H9724单克隆抗体呈阳性反应，对H6831单克隆抗体呈阴性反应，证实为莱姆病螺旋体。结论：山东省从长角血蜱中分离到莱姆病螺旋体尚属首次，且首次从病原学上证实山东省林区存在莱姆病疫源地。
Isolation and identification of Lyme disease spirochetes.METHODS: In 1996, in Tashan Forest Farm, Fei County, Shandong Province, the major ticks of Borrelia burgdorferi were investigated. A total of 900 ticks were collected by cloth flag method, all of them were H. longicornis. Results: Fifty spirochaetes were detected in midgut, with a rate of 12% (6/50). Two strains of spirochetes (TSH1 and TSH3) were isolated from 86 species (more than 850) of H. longicornis. The spirochete positive for H5332 and H9724 monoclonal antibodies, H6831 monoclonal antibody was negative, confirmed as Lyme disease spirochetes. Conclusion: It is the first time to isolate Lyme disease in Shandong Province, and it is the first time to etiologically confirm the existence of Lyme disease in the forest areas of Shandong Province.