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目的建立HPLC法同时测定肝力保胶囊中绿原酸和虎杖苷的含量。方法色谱柱为Agilent ZORBAX SBC18,流动相为0.1%甲酸-乙腈梯度洗脱,流速:1.0 ml·min-1,柱温:25℃,检测波长:327 nm,进样量5μl。结果绿原酸在1.365~68.25μg·ml-1(r=1.000)范围内线性关系良好,虎杖苷4.00~200.00μg·ml-1(r=1.000)范围内线性关系良好;日内和日间精密度均小于2%(n=3);绿原酸平均回收率为100.81%(RSD=1.74%,n=6),虎杖苷平均回收率99.87%(RSD=1.30%,n=6)。结论该法简便、结果准确、重复性好,可用于肝力保胶囊中绿原酸和虎杖苷的质量控制。
OBJECTIVE To establish a HPLC method for the simultaneous determination of chlorogenic acid and cediidin in Gengli capsule. Methods The column was Agilent ZORBAX SBC18. The mobile phase consisted of 0.1% formic acid-acetonitrile gradient. The flow rate was 1.0 ml · min-1. The column temperature was 25 ℃. The detection wavelength was 327 nm. Results The linearity of chlorogenic acid in the range of 1.365 ~ 68.25μg · ml-1 (r = 1.000) was good and the linear relationship was good in the range of 4.00 ~ 200.00μg · ml-1 (r = 1.000) The average recovery of chlorogenic acid was 100.81% (RSD = 1.74%, n = 6). The average recovery rate of polydatin was 99.87% (RSD = 1.30%, n = 6). Conclusion The method is simple, accurate, reproducible and can be used for the quality control of chlorogenic acid and cediid,