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多年来人们企图在原代、传代及双倍体细胞上来分离、传代流行性出血热病毒,均未获得成功。最近French和Lee等报道,他们已将朝鲜出血热病原因于适应到人肺癌A-549传代细胞(一株Ⅱ型肺泡上皮细胞)中。其方法是先用76/118株病毒第四代感染的黑线姬鼠的肺组织悬液,再感染黑线姬鼠后21天杀死,将其混合的肺组织制成10%悬液,然后用此悬液10~(-1)稀释度(用10%胎牛血清E-199液稀释)分别接种18种原代、双
Over the years, people tried to separate and pass epidemic hemorrhagic fever virus on primary, passage and diploid cells, but none succeeded. Recently, French and Lee reported that they have adapted the cause of the Korean hemorrhagic fever to human lung cancer A-549 passage cells, a type II alveolar epithelial cell. The method comprises the following steps: firstly, the lung tissue suspension of Apodemus agrarius infected with the fourth generation of 76/118 viruses is killed 21 days after being infected with Apodemus agrarius, the mixed lung tissue is made into a 10% suspension, Then, the suspension was diluted to 10 -1 (diluted with 10% fetal bovine serum E-199 solution) to inoculate 18 primary,