CD74在多种肠胃肿瘤中均有高水平的表达。为了探寻CD74表达与结肠癌的关系,Maharshak N、Cohen S、Lantner F等对正常小鼠结肠上皮细胞和结肠肿瘤细胞中CD74的表达及功能进行了深入的研究。采用RT-PCR、Western blotting及荧光激活细胞分类器(FACS)技术测量CD74基因和蛋白的表达。采用定量RT-PCR、RT-PCR、Western blotting及FACS技术研 CD74 is highly expressed in many gastrointestinal tumors. In order to explore the relationship between CD74 expression and colon cancer, Maharshak N, Cohen S, Lantner F and so on in normal mouse colon epithelial cells and colon tumor cells CD74 expression and function in-depth study. The expression of CD74 gene and protein was measured by RT-PCR, Western blotting and fluorescence activated cell classifier (FACS). Quantitative RT-PCR, RT-PCR, Western blotting and FACS techniques were used to investigate