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采用酶联免疫反应（ＥＬＩＳＡ）及聚合酶链反应（ＰＣＲ）方法，对２０例烧伤病人血清丙型肝炎病毒抗体（抗ＨＣＶ）及丙型肝炎病毒（ＨＣＶ）ＲＮＡ进行了检测。结果：入院时，输血组及非输血组挤－ＨＣＶ及ＨＣＶＲＮＡ检测均为阴性。一个月后，输血组１０例病人中发现抗－ＨＣＶ阳性５例、ＨＣＶ ＲＮＡ阳性４例；而非输血组抗－ＨＣＶ及ＨＣＶＲＮＡ检测均为阴性。说明输血是烧伤病人感染ＨＣＶ的重要途径。本文还对烧伤病人输血后抗－ＨＣＶ及ＨＣＶ ＲＮＡ阳性率高的原因进行了分析。
Serum anti-HCV and anti-HCV RNA were detected in 20 cases of burn patients by enzyme-linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR). Results: At hospital admission, both HCV-RNA and HCV RNA were negative in transfusion group and non-transfusion group. One month later, 5 patients were positive for anti-HCV and 4 patients were positive for HCV RNA in 10 patients in the blood transfusion group. The anti-HCV and HCV RNA in the non-blood transfusion group were all negative. Blood transfusion is an important way of HCV infection in burn patients. This article also analyzes the reasons for the high positive rate of anti-HCV and HCV RNA after transfusion in burn patients.