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目的探讨风湿康治疗大鼠膝骨关节炎的作用机制。方法膝关节腔注射4%的木瓜蛋白酶建立大鼠OA模型,1周后48只大鼠随机平均分成正常组、模型组、双醋瑞英组及1.35g/kg、2.7g/kg、5.4g/kg的风湿康治疗组共6组,正常组与模型组给与等体积的生理盐水。4周后处死动物,检测各组大鼠血清中IL-1β和TGF-β1的含量,切取大鼠膝关节,行大体和组织学观察,采用免疫组织化学方法检测基质金属蛋白酶-13(MMP-13)在关节软骨中的表达。结果风湿康治疗组均能降低血清中IL-1β的水平(P<0.001),风湿康中剂量和高剂量组能显著升高血清中TGF-β1的水平(P<0.001)。风湿康低剂量组对关节软骨的保护作用并不明显(P>0.05),风湿康中剂量和高剂量组均可显著抑制MMP-13在膝关节软骨中的表达(P<0.05),风湿康高剂量组与双醋瑞英组具有相似的疗效(P>0.05)。结论风湿康可能是通过降低血清中IL-1β、升高TGF-β1的水平、减少MMP-13在膝关节软骨中的表达,从而发挥其对骨关节炎的治疗作用。
Objective To investigate the mechanism of rheumatoid arthritis in treating knee osteoarthritis in rats. Methods OA rat models were established by injecting 4% papain into the knee joint cavity. One week later, 48 rats were randomly divided into normal group, model group, diacerebromine group and 1.35g / kg, 2.7g / kg, 5.4g / kg of rheumatism treatment group a total of 6 groups, normal group and model group given equal volume of saline. After 4 weeks, the animals were sacrificed and the contents of IL-1β and TGF-β1 in the serum of rats in each group were measured. The knee joints of rats were cut out for gross and histological observation. The expression of MMP- 13) Expression in Articular Cartilage. Results The rheumatoid arthritis treatment group decreased the level of IL-1β in serum (P <0.001), and the levels of TGF-β1 in serum increased significantly (P <0.001). Rheumatology and low dose group had no significant protective effect on articular cartilage (P> 0.05), while those in rheumatoid arthritis group and high dose group could significantly inhibit the expression of MMP-13 in articular cartilage (P <0.05) High-dose group and the two vinegar British group has a similar effect (P> 0.05). Conclusion It may be that rheumatoid may exert its therapeutic effect on osteoarthritis by decreasing IL-1β in serum, increasing the level of TGF-β1 and reducing the expression of MMP-13 in the articular cartilage.