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目的:建立石墨炉原子吸收分光光度法测定全血中锗的方法。方法:样品无需处理,以0.1%Tri-tonX-100/0.2%HNO3(v/v)为稀释液,以0.5 mg/ml硝酸钙为基体改进剂,对血液适当稀释后,直接用石墨炉原子吸收分光光度法测定锗的含量。结果:方法最低检出限(n=10)为0.75 mg/L,线性范围为0μg/L～190μg/L,相对标准偏差0.8%～3.2%,回收率为99.5%～103.6%。结论:方法具有简便、快速、回收率高、精密度高的优点,适用于全血中锗含量的测定。
Objective: To establish a method for the determination of germanium in whole blood by graphite furnace atomic absorption spectrophotometry. Methods: The samples were treated without any treatment. The samples were diluted with 0.1% Tri-ton X-100 / 0.2% HNO3 (v / v) and 0.5 mg / ml calcium nitrate as matrix modifier. Absorption spectrophotometry for the determination of germanium content. Results: The method showed the lowest detection limit (n = 10) was 0.75 mg / L, the linear range was 0μg / L ~ 190μg / L, the relative standard deviation was 0.8% ~ 3.2% and the recovery was 99.5% ~ 103.6%. Conclusion: The method is simple, rapid, high recovery, high precision, suitable for the determination of germanium in whole blood.