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目的探讨子宫内膜癌组织肿瘤坏死因子（ＴＮＦ）基因和蛋白及受体的表达及意义。②方法分别采用非同位素原位杂交方法、免疫组化方法对４８例子宫内膜癌组织ＴＮＦｍＲＮＡ，ＴＮＦ蛋白及ＴＮＦ受体（ＴＮＦＲ）的表达进行检测。③结果子宫内膜癌组织中１６例ＴＮＦｍＲＮＡ呈阳性表达，均为间质的单个核细胞和平滑肌细胞，腺癌细胞无阳性表达，对照组未见阳性表达。１５例病人ＴＮＦ蛋白呈阳性表达，２５例ＴＮＦＲ呈阳性表达，对照组子宫内膜腺体也有ＴＮＦＲ阳性表达，两组比较差异无显著性（χ２＝２．４５，Ｐ＞０．０５）。ＴＮＦｍＲＮＡ与ＴＮＦ蛋白表达存在不一致性，ＴＮＦ与ＴＮＦＲ表达与组织学分化及肌层浸润深度差异均无显著性（χ２＝２．１７，０．８１，Ｐ＞０．０５）；ＴＮＦＲ蛋白表达明显高于ＴＮＦ，两者比较差异有极显著性（χ２＝４．２９，Ｐ＜０．０５）；ＴＮＦＲ在正常内膜表达与子宫内膜癌表达差异无显著性（χ２＝２．４５，Ｐ＞０．０５）。④结论子宫内膜癌间质细胞能够表达ＴＮＦｍＲＮＡ，并产生ＴＮＦ蛋白。
Objective To investigate the expression and significance of tumor necrosis factor (TNF) gene and protein and its receptor in endometrial carcinoma. Methods The expression of TNF mRNA, TNF protein and TNF receptor (TNFR) in 48 cases of endometrial carcinoma were detected by non-isotopic in situ hybridization and immunohistochemistry respectively. Results The expression of TNFmRNA in 16 cases of endometrial carcinoma was positive, both of which were interstitial mononuclear cells and smooth muscle cells. No positive expression was found in adenocarcinoma cells and no positive expression in control group. The TNF protein of 15 patients was positive, 25 TNFR was positive, while that of control group was TNFR. There was no significant difference between the two groups (χ2 = 2.45, P> 0.05). The expression of TNF mRNA and TNF protein were not consistent, the expression of TNF and TNFR was not significantly different from the histological differentiation and the depth of myometrial invasion (χ2 = 2.17,0.81, P> 0.05); TNFR protein expression was significantly higher (Χ2 = 4.29, P <0.05). There was no significant difference in the expression of TNFR between normal endometrium and endometrial carcinoma (χ2 = 2.45, P> 0.05). ④ Conclusion Endometrial stromal cells can express TNF mRNA and produce TNF protein.