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报道了斑点免疫吸附ＥＬＩＳＡ（Ｄｏｔ－ＥＬＩＳＡ）快速检测草鱼出血病病毒（ＧＣＨＶ）的方法以及对提纯病毒、染毒细胞内病毒和病鱼组织的检测结果，并对该方法的灵敏度与ＳＰＡ－ＣｏＡ和常规ＥＬＩＳＡ法进行了系统的比较。结果表明，Ｄｏｔ－ＥＬＩＳＡ检出ＧＣＨＶ的最低含量为３ｐｇ，该灵敏度比ＳＰＡ－ＣｏＡ和常规ＥＬＩＳＡ法分别提高１０倍和２０倍，而且快速易行，是目前检测ＧＣＨＶ最为有效的方法，并可在草鱼出血病临床诊断和病毒疫苗质量鉴定等方面得到应用。
The method for rapid detection of GCHV by Dot-ELISA and the detection results of the purified virus, virus-infected intracellular virus and diseased fish tissues were reported. The sensitivity of this method to that of SPA-CoA And conventional ELISA method for a systematic comparison. The results showed that the minimum detectable level of GCHV by Dot-ELISA was 3 pg, which was 10-fold and 20-fold higher than that of SPA-CoA and conventional ELISA, respectively. Moreover, it was the most effective method to detect GCHV currently. Grass carp hemorrhage clinical diagnosis and virus vaccine quality identification and other aspects of the application.