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目的 观察不同剂量二硫化碳 (CS2 )对血管内皮细胞的损伤作用及维生素C的拮抗作用。方法 分别以不同浓度CS2 对培养的人脐带静脉内皮细胞进行染毒实验 ,同时以不同浓度维生素C预处理进行干预试验。采用MTT法分析细胞增殖活性 ,用二苯胺法进行细胞DNA断裂的定量分析。结果 CS2 可抑制脐静脉内皮细胞增殖活性 ,随着CS2 染毒浓度升高 ,内皮细胞增殖活性逐步下降 ,呈剂量反应关系 ;CS2 可致内皮细胞DNA损伤 ,使DNA双链发生断裂。浓度为 2 0 μmol ml时即可产生明显影响 ,浓度达到 16 0 μmol ml,细胞DNA完整率下降至 4 8.2 6 %。经维生素C预处理后 ,内皮细胞DNA完整率高于未经预处理组 ,差异有显著性 (P <0 .0 1) ,增殖活性也有明显改善。结论 CS2对脐静脉内皮细胞有直接的损伤作用 ,而维生素C可拮抗CS2 致脐静脉内皮细胞损伤作用。
Objective To observe the effects of different doses of carbon disulfide (CS2) on the injury of vascular endothelial cells and the antagonism of vitamin C. Methods Cultured human umbilical vein endothelial cells were exposed to different concentrations of CS2, respectively. At the same time, different concentrations of vitamin C pretreatment were used for intervention experiments. The cell proliferation activity was analyzed by MTT assay and the DNA fragmentation was quantitatively analyzed by diphenylamine method. Results CS2 inhibited the proliferation of human umbilical vein endothelial cells. With the increase of concentration of CS2, the proliferative activity of endothelial cells gradually decreased and showed a dose-response relationship. CS2 induced DNA damage in endothelial cells and ruptured the double-stranded DNA. The concentration of 20 μmol ml can produce significant effects, the concentration of 16 0 μmol ml, cell DNA integrity decreased to 4 8.26%. After pretreatment with vitamin C, the DNA integrity rate of endothelial cells was higher than that of the non-pretreated group (P <0.01), and the proliferative activity also remarkably improved. Conclusion CS2 has a direct injury on umbilical vein endothelial cells, while vitamin C can antagonize the injury of umbilical vein endothelial cells induced by CS2.