选雄性Wistar大鼠(体重250 g),研究铅对肝微粒体的药物代谢和骨髓的卟啉代谢的生物学效应。急性试验用5%葡萄糖液配制醋酸铅,腹腔注入每公斤体重0(对照)、0.1、1.0、10和50 mg,每天一次,连续3天。慢性试验腹腔注入每公斤体重0(对照)、5和20 mg,每周一次,连续9周,共10次。在最后一次注射后,要防止大鼠吃饲料笼中的饲料碎片和粪渣,以保证每只大鼠在笼中禁食22小时,然后处死备用。大鼠的肝脏微粒体酶主要用于评价铅对肝功能的影响。急性试验结果表明,铅降低了药物代谢酶的活性,如苯胺水解酶和氨基比林脱甲基酶,还降低了微粒体中细胞色素P_(450)和细胞色素b_5的 Male Wistar rats (body weight 250 g) were selected to study the biological effects of lead on the metabolism of liver microsomes and the porphyrin metabolism of bone marrow. Acute Test Lead acetate was formulated in 5% dextrose solution and injected intraperitoneally at 0 (control) per kg body weight, 0.1, 1.0, 10 and 50 mg once daily for 3 consecutive days. Chronic intraperitoneal injection of 0 (CK) per kilogram of body weight, 5 and 20 mg once weekly for 9 weeks, 10 times in total. After the last injection, rats should be prevented from eating feed pellets and dung in the feed cage to ensure that each rat is fasted for 22 hours in the cage and then sacrificed for later use. Rat liver microsomal enzymes are mainly used to evaluate the impact of lead on liver function. Acute experimental results show that lead reduces the activity of drug metabolizing enzymes, such as aniline hydrolase and aminopyrine demethylase, and also reduces the cytochrome P_ (450) and cytochrome b_5