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本文建立了测定左旋吡喹酮血药浓度的高效液相色谱分析方法.以ODS-C_(18)化学键合相(5μm)作为固定相,甲醇:水(7:3V/V)作为流动相;用吡喹酮的衍生物作为内标准品.采用紫外检测器(λ=220nm),得出检出限为2.5ng,最低检出血药浓度为0.012μg/ml血清,线性范围为0.012～1.6μg/ml血清(γ=0.999),方法回收率为93.4～97.2%.本法简便、灵敏、专一、重现性好,适用于左旋吡喹酮血药浓度测定及药物动力学研究
A high performance liquid chromatographic method for the determination of the concentration of L-praziquantel was established in this paper. Methanol: water (7: 3 V / V) was used as mobile phase with ODS-C 18 chemically bonded phase Using praziquantel derivatives as the internal standard, the detection limit was 2.5ng with the UV detector (λ = 220nm), the minimum detectable serum concentration was 0.012μg / ml, the linear range was 0.012 ~ 1.6 μg / ml serum (γ = 0.999), the recovery rate was 93.4 ~ 97.2% .The method was simple, sensitive, specific and reproducible.It was suitable for the determination of the concentration of the drug and its pharmacokinetics