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目的研究银杏叶提取物用于衰老大鼠模型的血清指纹图谱,探讨其体内药效成分。方法建立D-半乳糖致衰老大鼠模型,同时ig给予银杏叶提取物,制备衰老大鼠的含药血清,采用HPLC-UV和HPLC-ESI-MS法,分别针对黄酮类成分和内酯类成分建立指纹图谱,分析入血成分。结果 HPLC-UV指纹图谱显示了10个入血成分,大多为代谢成分;HPLC-MS指纹图谱标定了9个共有峰,其中7个为原型成分。结论首次进行了银杏叶提取物的血清药物成分图谱研究,可反映银杏叶提取物口服给药吸收入血药物成分的变化,为其体内药效物质研究奠定基础。
Objective To study the serum fingerprint of Ginkgo biloba extract for aging rat model and to explore its pharmacodynamic components. Methods A rat model of aging induced by D-galactose was established. At the same time, Ginkgo biloba extract was applied to prepare senile rat serum. HPLC-UV and HPLC-ESI-MS methods were used to detect flavonoids and lactones Composition Fingerprinting, analysis of blood components. Results HPLC-UV fingerprinting showed that 10 blood components were mostly metabolites. HPLC-MS fingerprinting identified 9 common peaks, of which 7 were prototype components. Conclusions The study of serum components of Ginkgo Biloba extract for the first time can reflect the changes of drug components of oral administration of Ginkgo biloba extract absorbed into the blood and lay a foundation for the study of its pharmacodynamic substances in vivo.