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目的从海茸(Durvillaea antarctica)中提取分离多糖并对其结构进行表征。方法采用冷水提取,然后用离子交换色谱柱分离纯化得到多糖HRC0和HRC1,用高效凝胶渗透色谱法(HPGPC)测定相对分子质量,用高效液相色谱法(HPLC)测定单糖组成,红外光谱(IR)和核磁共振波谱法(~1H-NMR,~(13)C-NMR)对其化学结构进行表征。结果 HRC0是一种相对分子质量为127.9 kD的线型β-1,3-葡聚糖;HRC1是一种相对分子质量为208.1 kD,且甘露糖醛酸含量高达83%的褐藻胶。结论海茸冷水提取物中主要是β-1,3-葡聚糖和高甘露糖醛酸含量的褐藻胶。
Objective To isolate and characterize the polysaccharides of Durvillaea antarctica. Methods Cold water extraction was used. The polysaccharides HRC0 and HRC1 were separated and purified by ion exchange chromatography. The relative molecular mass was determined by high performance liquid chromatography (HPGPC). The content of monosaccharide was determined by high performance liquid chromatography (HPLC) (IR) and nuclear magnetic resonance spectroscopy (~ 1H-NMR, ~ (13) C-NMR). Results HRC0 was a linear β-1,3-glucan with a relative molecular mass of 127.9 kD. HRC1 was an alginate with a relative molecular mass of 208.1 kD and a mannuronic acid content of 83%. Conclusion The brown seaweed extract is mainly β-1,3-glucan and high mannuronic acid content alginate.