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目的 观察半胱氨酸天冬氨酸酶3（caspase 3）mRNA在RCS大鼠视网膜中的表达,分析caspase 3特异抑制剂 Ac-DEVD-CHO对视细胞凋亡的影响。方法 应用逆转录-多聚酶链反应（RT-PCR）检测RCS大鼠出生后不同时间视网膜中caspase3 mRNA的表达。应用TUNEL方法检测玻璃体内注射Ac-DEVD-CHO后视细胞凋亡的改变。结果RCS大鼠出生后14天至60天视网膜 caspase 3 mRNA均有表达,25天水平明显升高,达到高峰。SD大鼠视网膜15至60天的表达量无明显差异,与RCS大鼠14天水平接近。caspase 3特异抑制剂 Ac-DEVD-CHO能显著抑制视细胞凋亡。结论 caspase 3可能在 RCS大鼠视网膜变性视细胞凋亡中起关键作用。
Objective To observe the expression of caspase 3 mRNA in the retina of RCS rats and the effect of Ac-DEVD-CHO, a specific inhibitor of caspase 3, on the apoptosis of retinal cells. Methods Reverse transcription polymerase chain reaction (RT-PCR) was used to detect the expression of caspase3 mRNA in retina of RCS rats at different time after birth. TUNEL method was used to detect the change of apoptotic cells after intravitreal injection of Ac-DEVD-CHO. Results The expression of caspase 3 mRNA in the retina of RCS rats after 14 days to 60 days of birth was significantly increased, reaching a peak at 25 days. There was no significant difference in the expression of Retina between 15 and 60 days in SD rats, which was similar to that of RCS rats at 14 days. Ac-DEVD-CHO, a specific inhibitor of caspase 3, significantly inhibited the apoptosis of optic nerve cells. Conclusion Caspase 3 may play a key role in retinal degeneration in RCS rats.