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目的探讨合肥市输入性恶性疟原虫Pfcrt基因K76T位点的突变情况。方法采集2010年从非洲疟疾高流行区,务工回国输入性恶性疟病人血样11份,采用恶性疟原虫Pfcrt基因序列特异引物,以滤纸血片中的恶性疟原虫DNA为模板,进行巢氏PCR扩增基因,扩增产物经限制性内切酶酶切后鉴定。结果收集到11例滤纸血样,成功扩增6例,其中2例被酶切,为Pfcrt等位基因野生型;4例未被酶切,为Pfcrt等位基因突变型。结论合肥市输入性恶性疟中Pfcrt等位基因发生突变,应该加强输入性恶性疟的药物抗性监测。
Objective To investigate the mutation of Pfcrt gene K76T in Plasmodium falciparum in Hefei. Methods A total of 11 blood samples of falciparum malaria patients were imported from malaria-endemic areas in Africa in 2010. Plasmodium falciparum DNA in filter paper was used as a template for Pseudomonas falciparum Pfcrt gene sequence-specific PCR. By gene, amplification products were identified by restriction endonuclease digestion. Results The blood samples of 11 filter papers were collected and 6 cases were successfully amplified. Two of them were digested with Pfcrt allele. Four of them were not digested and were Pfcrt allelic mutant. Conclusion The mutation of Pfcrt allele in imported falciparum malaria in Hefei City should be strengthened, and drug resistance monitoring of imported falciparum malaria should be strengthened.