论文部分内容阅读
目的:研究胃肠舒和血流干预药物对糖尿病胃轻瘫大鼠胃窦平滑肌细胞(SMC)病变的影响。方法:131只大鼠,随机分为正常组(10)、模型组(22)、止血敏组(33)、蚓激酶组(33)和胃肠舒组(33),除正常组外大鼠1次性ip链脲佐菌素(60 mg.kg-1)造模,以7天后血糖≥16.7 mmol.L-1为纳入标准。3天后开始分别ig胃肠舒、止血敏i、p蚓激酶,模型组和正常组予蒸馏水ig。30周后检测血糖、碘[125I]-胰岛素(FINS)、碘[125I]-C肽(C-P)、胃液体排空率等。取胃窦组织,行HE染色、Mallory染色和免疫组化染色,光镜下观察胃窦组织SMC形态变化、SMC凋亡以及凋亡调控基因Bcl-2和Fax基因蛋白的表达。电镜下观察胃窦组织SMC超微结构改变。结果:30周后模型组大鼠胃窦SMC变性,SMC凋亡指数升高,与正常组比较差异显著(P<0.01),SMC中Bcl-2基因蛋白表达减低、Fas基因蛋白过度表达,与正常组比较有显著性差异(P<0.01)。胃肠舒和蚓激酶治疗后胃窦平滑肌病变明显改善,而止血敏却使胃窦平滑肌病变加重。结论:糖尿病胃轻瘫存在着胃窦SMC病变,血流干预药物对其有影响,胃肠舒具有降糖、改善胃窦SMC病变、促进胃动力的作用。
OBJECTIVE: To study the effects of Weichangshu and blood flow interfering drugs on gastric smooth muscle cell (SMC) lesions in diabetic gastroparesis rats. METHODS: 131 rats were randomly divided into normal group (10), model group (22), hemostasis-sensitive group (33), lumbrokinase group (33), and Weichangshu group (33). Rats were excluded from normal group. One-time IP streptozotocin (60 mg.kg-1) was modeled, and blood glucose ≥16.7 mmol.L-1 was included as an inclusion criteria after 7 days. After 3 days, ig shu shu, shizhi mei i, and p kin kinase were started respectively. Distilled water was ig in the model group and the normal group. After 30 weeks, blood glucose, iodine [125I]-insulin (FINS), iodine [125I]-C peptide (C-P), and gastric emptying rate were measured. Gastric antrum tissues were collected and stained with HE, Mallory, and immunohistochemistry. Morphological changes of SMC in gastric antrum, apoptosis of SMC, and expression of apoptosis-regulating genes Bcl-2 and Fax were observed under light microscope. The SMC ultrastructure was observed under electron microscope. RESULTS: After 30 weeks, the gastric antrum SMC degeneration in the model group and the apoptosis index of SMC increased. There was a significant difference compared with the normal group (P<0.01). The expression of Bcl-2 gene protein was decreased and the Fas gene protein was over-expressed in SMCs. There was a significant difference in the normal group (P<0.01). Gastrointestinal sinokinase and lumbrokinase treatment significantly improved the gastric antrum smooth muscle lesions, while hemostatic sensitization resulted in aggravation of gastric antrum smooth muscle lesions. Conclusions: Diabetic gastroparesis has gastric antral SMC lesions, which are influenced by blood flow intervention drugs. Gastrointestinal tract has hypoglycemic effect, improves antral SMC lesions, and promotes gastric motility.