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目的观察柿叶黄酮(PLF)对阿霉素肾病大鼠肾组织金属蛋白酶抑制物1(TIMP-1)表达及对肾组织病理损伤的影响。方法制备阿霉素肾病大鼠模型及药物干预模型鼠,测定模型鼠24 h尿蛋白(24hUP)变化,用免疫组化法检测肾组织中TIMP-1表达,光学显微镜观察肾组织病理形态。结果 PLF能明显降低阿霉素肾病大鼠24hUP(P<0.01),减轻肾脏免疫病理损伤及抑制肾组织TIMP-1的表达(P<0.01)。结论 PLF能降低模型鼠肾组织TIMP-1的表达、抑制肾组织纤维化等,对阿霉素肾病大鼠肾脏起到保护作用。
Objective To observe the effect of persimmon leaf flavonoid (PLF) on the expression of tissue inhibitor of metalloproteinase 1 (TIMP-1) in renal tissue of rats with adriamycin-induced nephropathy and the pathological changes of renal tissue. Methods The adriamycin-induced nephropathy rat model and drug-intervention model rats were established. 24-hour urine protein (24hUP) was measured. The expression of TIMP-1 in renal tissue was detected by immunohistochemistry and the pathological changes of renal tissue were observed by light microscopy. Results PLF could significantly reduce adriamycin-induced nephropathy rats 24hUP (P <0.01), reduce renal immune pathological damage and inhibit renal tissue TIMP-1 expression (P <0.01). Conclusion PLF can reduce the expression of TIMP-1 in renal tissue of rats and inhibit the renal fibrosis, which may play a protective role on the kidneys of adrimycin-induced nephrotic rats.