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目的:探讨长链非编码RNA(long non-coding RNA,lncRNA)肌动蛋白纤维相关蛋白-1反义RNA1(actin filamentassociated protein 1-antisense RNA1,AFAP1-AS1 RNA1)在胃癌组织中的表达情况及其临床意义。方法:采用Real-time PCR方法检测2010年1月至2014年12月宜昌市第二人民医院及三峡大学仁和医院手术切除的274例胃癌组织及相应癌旁正常胃组织中AFAP1-AS1的表达,并分析AFAP1-AS1表达量与临床及病理特征的关系。结果:AFAP1-AS1在胃癌组织比癌旁正常组织显著高表达(P<0.01)。AFAP1-AS1在早期胃癌组织中平均表达量明显低于进展期胃癌(P<0.01)。在不同病理类型胃癌中,AFAP1-AS1的表达量没有差异(P>0.05)。AFAP1-AS1表达量和胃癌淋巴侵袭或远处转移密切相关,在伴有淋巴结侵袭或远处转移的胃癌组织中,AFAP1-AS1明显高表达(P<0.01)。结论:AFAP1-AS1可能是胃癌发生发展的一个重要因素;有望成为新的胃癌预后标志物,用于胃癌的临床诊断和预后判断。
AIM: To investigate the expression of long non-coding RNA (siRNA) 1 antisense RNA1 and AFAP1-AS1 RNA1 in gastric cancer tissues and its significance Its clinical significance. Methods: Real-time PCR was used to detect the expression of AFAP1-AS1 in 274 cases of gastric cancer tissues and corresponding normal gastric tissues from January 2010 to December 2014 in Yichang Second People’s Hospital and Renhe Hospital of China Three Gorges University. And analyze the relationship between AFAP1-AS1 expression and clinical and pathological features. Results: AFAP1-AS1 was significantly higher in gastric cancer tissues than normal tissues (P <0.01). The average expression of AFAP1-AS1 in early gastric cancer was significantly lower than that in advanced gastric cancer (P <0.01). In different pathological types of gastric cancer, AFAP1-AS1 expression was no difference (P> 0.05). The expression of AFAP1-AS1 is closely related to lymph node invasion or distant metastasis of gastric cancer, and AFAP1-AS1 is highly expressed in gastric cancer tissues with lymph node invasion or distant metastasis (P <0.01). Conclusion: AFAP1-AS1 may be an important factor in the development of gastric cancer. It is expected to become a new prognostic marker of gastric cancer for clinical diagnosis and prognosis of gastric cancer.