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通过对不同产地杜仲叶中次生代谢物（ 有效成分）含量的测定及其生态因素分析，对杜仲叶次生代谢物进行了生态学研究，结果表明：不同产地杜仲叶次生代谢物含量差异显著：遵义地区的京尼平甙、绿原酸、桃叶栅瑚甙、总黄酮含量最高，慈利地区的京尼平甙酸含量最高；洛阳地区的京尼平甙含量最低，宜昌地区的绿原酸、桃叶珊瑚甙、总黄酮、京尼平甙酸含量最低，但杜仲胶含量最高，从而说明了生态因素也是调控次生代谢物的重要因素。对各生态因子进行逐步回归分析和通径分析的结果表明：影响杜仲叶次生代谢物合成与积累的主要因素是微量元素；土壤中微量元素（ 有效态） 与杜仲（ 叶） 中微量元素（ 植物吸收） 不完全呈正相关关系；以两者为主因素各进行的逐步回归分析和通径分析的结果不一致。通过系统聚类分析和模糊综合评判得出：贵州遵义、湖南慈利、陕西略阳、四川通江４ 个地区杜仲叶中次生代谢物含量较高
The secondary metabolites of Eucommia ulmoides leaves were studied by measuring the content of secondary metabolites (active ingredients) and their ecological factors in different origins of Eucommia ulmoides leaves. The results showed that the secondary metabolites of Eucommia ulmoides leaves were significantly different: Zunyi Geniposide, chlorogenic acid, peach cashew glucoside, the highest content of total flavonoids, geniposide in Cili the highest content of geniposidic acid; Luoyang genipin content lowest, Yichang area of chlorogenic acid, The content of aucubin, total flavonoids and geniposidic acid was the lowest, but the content of Eucommia ulmoides gum was the highest, indicating that the ecological factors are also important factors in the regulation of secondary metabolites. The results of stepwise regression analysis and path analysis showed that the main factors influencing the synthesis and accumulation of secondary metabolites in Eucommia ulmoides leaves were trace elements; the contents of trace elements (available) in soil and the contents of trace elements ) Is not completely positive correlation; both the main factors of the progressive regression analysis and path analysis of the results are inconsistent. The results of cluster analysis and fuzzy comprehensive evaluation showed that the secondary metabolites of Eucommia ulmoides leaves were higher in Zunyi of Guizhou, Cili of Hunan, Lueyang of Shaanxi and Tongjiang of Sichuan.