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目的探讨γ干扰素(IFN-γ)对甲状腺细胞分泌趋化因子的作用及机制。方法 HE染色检测桥本甲状腺炎组织中淋巴细胞浸润情况,免疫组织化学染色检测IFN-γ的表达。用500 U/mL IFN-γ处理Nthy-ori 3-1甲状腺细胞后,利用Western blot法检测信号转导子及转录激活子3(STAT3)及磷酸化的STAT3的水平。用STAT3抑制剂Stattic处理后,ELISA测定CXC趋化因子配体10(CXCL10)的变化,TranswellTM法检测淋巴细胞的迁移能力。结果与正常甲状腺组织比较,桥本甲状腺炎组织中淋巴细胞浸润较多,IFN-γ表达水平较高。与对照组比较,IFN-γ处理增加甲状腺细胞p-STAT3水平,增加CXCL10分泌和淋巴细胞迁移,而Stattic处理后CXCL10分泌减少,淋巴细胞迁移减少。结论 IFN-γ激活STAT3促进甲状腺上皮细胞分泌趋化因子,增加淋巴细胞的迁移。
Objective To investigate the effect and mechanism of γ interferon (IFN-γ) on the secretion of chemokines from thyroid cells. Methods Lymphocyte infiltration in Hashimoto’s thyroiditis was detected by HE staining and the expression of IFN-γ was detected by immunohistochemical staining. After Nthy-ori 3-1 thyroid cells were treated with 500 U / mL IFN-γ, the levels of signal transducer and activator of transcription 3 (STAT3) and phosphorylated STAT3 were detected by Western blot. After treatment with STAT3 inhibitor Stattic, the changes of CXC chemokine ligand 10 (CXCL10) were detected by ELISA, and the migration ability of lymphocytes was detected by TranswellTM. Results Compared with normal thyroid tissue, Hashimoto’s thyroiditis had more lymphocyte infiltration and higher IFN-γ expression. Compared with the control group, IFN-γ treatment increased p-STAT3 levels in thyrocytes, increased CXCL10 secretion and lymphocyte migration, while decreased CXCL10 secretion and decreased lymphocyte migration after Stattic treatment. Conclusion IFN-γ activates STAT3 to promote the secretion of chemokines by thyroid epithelial cells and increase the migration of lymphocytes.