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用细胞因子活性检测，Ｎｏｒｔｈｅｒｎｂｌｏｔ方法，在体外研究了抗细菌核心糖脂域ＭｃＡｂ（ＥＬ１、ＥＬ３和３Ｈ４）对ＬＰＳ诱导人外周血单核细胞（ｈＰＢＭＣ）释放ＴＮＦ－α和ＩＬ－６及其ｍＲＮＡ表达水平的影响。结果表明，ＥＬ１、ＥＬ３和３Ｈ４在体外有抑制ＬＰＳ诱导细胞释放ＴＮＦ－α和ＩＬ－６的作用；Ｎｏｒｔｈｅｒｎｂｌｏｔ结果证实，这３株ＭｃＡｂ能分别降低细胞ＴＮＦ－α和ＩＬ－６ｍＲＮＡ表达的水平；提示抗细菌核心糖脂域ＭｃＡｂ可能通过与ＬＰＳ的结合而中和ＬＰＳ，从而降低或阻碍了效应细胞炎性细胞因子ｍＲＮＡ的表达，达到降低相应细胞因子的作用。
Cytokine activity assay and Northern blot method were used to study the effects of antibacterial core glycolipid McAbs (EL1, EL3 and 3H4) on the release of TNF-α, IL-6 and its mRNA from human peripheral blood mononuclear cells (hPBMC) induced by LPS Effect of expression level. The results showed that EL1, EL3 and 3H4 could inhibit the release of TNF-α and IL-6 from LPS-induced cells in vitro. The results of Northern blot showed that the three McAbs could reduce the expression of TNF-α and IL-6 mRNA, respectively Antibacterial core glycolipid McAb may neutralize LPS by binding to LPS, so as to reduce or block the expression of effector cell inflammatory cytokine mRNA to reduce the corresponding cytokine.