在4种培养系统中,马来丝虫Ⅲ期幼虫在改良RPMI1640、20%小牛血清和人胚肾细胞系为饲养层的培养系统中生长发育良好,可以从Ⅲ期幼虫蜕皮2次发育到童虫,最长存活时间为54d。在单纯改良RPMI1640、TC199和20％小牛血清中培养,从Ⅲ期幼虫发育到童虫的最长存活时间为42d。班氏丝虫Ⅲ期幼虫在上述2种培养系统中,分别存活57和36d,从Ⅲ期幼虫蜕皮进入Ⅳ期幼虫和童虫。对马来丝虫幼虫体外培养蜕皮时间、虫体大小与沙鼠体内发育情况进行了比较。 Among the four culture systems, the third stage larvae of M. malayi grew well in the culture system of modified RPMI1640, 20% calf serum and human embryonic kidney cell line as feeder layer, Squirrels, the longest survival time of 54d. In pure modified RPMI1640, TC199 and 20% fetal bovine serum, the longest survival time from stage III larvae to schistosomula was 42 days. Bancroftian filarial stage III larvae survived for 57 and 36 days, respectively, in the two culture systems described above, and entered stage IV larvae and snails from stage III larva molting. Malayan larvae cultured in vitro molting time, body size and development in vivo were compared.