论文部分内容阅读
目的:建立蒙药材大托叶云实的质量标准,为控制和评价该药材质量提供有效的实验依据.方法:采用显微鉴别法对大托叶云实的显微特征进行描述.参照2015年版《中国药典》中相关方法,对大托叶云实药材灰分进行测定.以齐墩果酸为对照品,香草醛-高氯酸为显色剂,于548nm波长处,采用紫外可见分光光度法对7个批次的大托叶云实中的总三萜类化合物含量进行测定.结果:7批次大托叶云实总灰分平均含量为4.802％,在选定的最优条件下,总三萜类化合物在0.004～0.024mg/mL范围内线性关系良好,线性回归方程为y=54.914x+0.0029,相关系数r=0.999,平均加样回收率为98.78％,RSD 为1.2％,7批次大托叶云实中总三萜类化合物的含量为0.287％～0.346％.结论:建立的大托叶云实性状、显微鉴别法操作简单,专属性较强,能有效评价和控制其内在质量和用药品质.“,”Objective:To establish quality standards of Mongolian medicine Caesalpiniae cristae semen in order to provide effective experiment basis for control and evaluation of Mongolia medicine quality.Methods:Microscopic characteristic of Caesalpiniae cristae semen was described firstly in this study.Then,the contents of total ash were analyzed according to Chinese Pharmacopoeia(2015 version).Finally,we applied oleanolic acid as reference substance,and vanillin and pleanolic acid for color reaction,then the content of triterpenoids in Caesalpiniae cristae semen was determined by Ultraviolet visible spectrophotometer at 548nm.Results:The average content of total ash was 4.802％,had shown a better linearity within the range of 0.004～0.024mg/mL,with the linearity equation of y=54.914x+0.0029(r=0.999),Average recovery of spiked sample was 98.78％and RSD％ was 1.2％.The assay contents of 7batches were the range of 0.287％～0.346％.Conclusion:The established methods of morphological characters and microscopic identification were simple to operate and have strong specificity.The quality standards established on the basis of the above results were suitable for the quality evaluation of Caesalpiniae cristae semen.