通过比较浸叶法、药膜法和Potter喷雾法3种生测方法对螺旋粉虱成虫进行生物测定,确定了喷雾法是螺旋粉虱适宜的生测方法。优化了Potter喷雾法的操作因子,当喷雾量为1~2 m L、喷雾压力为50~100 k Pa、沉降时间为20~40 s时,Potter喷雾法的测定结果更准确、可靠。确证了采用螺旋粉虱自然混合日龄成虫作为试虫的可靠性。该方法可作为螺旋粉虱药剂筛选及抗药性监测的生物测定方法。 The bioassay of adults of B. tabaci was carried out by comparing the dipping method, the membrane method and the Potter spray method. It was confirmed that the spray method was a suitable method for the bioassay of the B. tabaci. Potter spray method was optimized for operating factors. Potter spray method was more accurate and reliable when the spray volume was 1 ~ 2 m L, the spray pressure was 50 ~ 100 kPa, and the settling time was 20 ~ 40 s. Confirmed the use of natural whitefly mixed day-old adults as test insects reliability. The method can be used as a bioassay method for drug screening and drug resistance monitoring of whitefly.