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Objective In this study, we evaluated the diagnostic efficiency of six recombinant proteins for the serodiagnosis of Lyme borreliosis(LB) and screened out the appropriate antigens to support the production of a Chinese clinical ELISA(enzyme-linked immunosorbent assay) kit for LB. Methods Six recombinant antigens, Fla B.g, Osp C B.a, Osp C B.g, P39 B.g, P83 B.g, and Vls E B.a, were used for ELISA to detect serum antibodies in LB, syphilis, and healthy controls. The ELISA results were used to generate receiver operating characteristic(ROC) curves, and the sensitivity and specificity of each protein was evaluated. All recombinant proteins were evaluated and screened by using logistic regression models. Results Two Ig G(Vls E and Osp C B.g) and two Ig M(Osp C B.g and Osp C B.a) antigens were left by the logistic regression model screened. Vls E had the highest specificity for syphilis samples in the Ig G test(87.7%, P<0.05). Osp C B.g had the highest diagnostic value in the Ig M test(AUC=0.871). Interactive effects between Osp C B.a and Fla B.g could reduce the specificity of the ELISA. Conclusion Three recombinant antigens, Osp C B.g, Osp C B.a, and Vls E B.a, were useful for ELISAs of LB. Additionally, the interaction between Osp C B.a and Fla B.g should be examined in future research.
Objective In this study, we evaluated the diagnostic efficiency of six recombinant proteins for the serodiagnosis of Lyme borreliosis (LB) and screened out the appropriate antigens to support the production of a Chinese clinical ELISA (enzyme-linked immunosorbent assay) kit for LB. Methods Six recombinant antigens, Fla Bg, Osp C Ba, Osp C Bg, P39 Bg, P83 Bg, and Vls E Ba, were used for ELISA to detect serum antibodies in LB, syphilis, and healthy controls. The ELISA results were used to generate Both recombinant proteins were evaluated and screened by using logistic regression models. Results Two Ig G (Vls E and Osp C Bg) and two Ig M (Osp Cgg and Osp C Ba) antigens were left by the logistic regression model screened. Vls E had the highest specificity for syphilis samples in the Ig G test (87.7%, P <0.05). Osp C Bg had the highest diagnostic value in the Ig M test (AUC = 0 .871). Interactive effects between Osp C Ba and Fla Bg could reduce the specificity of the ELISA. Three Recombinant Antigens, Osp C Bg, Osp C Ba, and Vls E Ba, were useful for ELISAs of LB. between Osp C Ba and Fla Bg should be examined in future research.