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目的为进一步认识、探讨三七有效成分Rx抗心肌缺血再灌注损伤的保护作用机理提供实验理论基础。方法利用改良的Langendorff灌流装置建立离体大鼠心脏缺血再灌注损伤动物实验模型,分别用三七有效成分Rx灌流液小剂量5mg/L,中剂量10mg/L,大剂量20mg/L,异搏定400μmol/L,分别再灌注缺血离体心脏,从血流动力学(LVP、LVEP、CRP、HR),抗氧自由基(LPO),心脏能量代谢(ATP、ADP、AMP、CAMP),心肌损伤程度(LDH、CPK),血管内皮活性因子(TXB2、PGF1α)变化等方面观察三七有效成分不同剂量组与阳性药物异搏定及模型组比较观察。结果该药对血流动力学中指标LVP增加作用(P<0.05),LVEP、CRP、HR有下降作用(P<0.05);对LPO产生有减少作用(P<0.05);能量代谢中ATP、CAMP含量增加(P<0.05),ADP、AMP含量减少(P<0.05);对LDH、CPK产生减少(P<0.05);TXB2的释放减少(P<0.05),PGF1α的含量增加(P<0.05);各作用随药物剂量的增加而增强。结论三七有效成分Rx能增强心肌收缩力,减低心率,增加心输出量,抗氧自由基的产生,减轻心肌耗能,减轻心肌损伤程度,降低LDH、CPK的释放,促进内皮活性因子的释放,保护内皮细胞;且剂量与效应成正比关系。提示三七有效成分Rx对离体心脏缺血再灌注损伤影响具有保护作用。
Objective To provide a theoretical basis for further understanding and exploring the protective mechanism of Rx, an effective component of Panax notoginseng, against myocardial ischemia-reperfusion injury. Methods A modified Langendorff perfusion device was used to establish an animal model of isolated rat heart ischemia-reperfusion injury. The active ingredients of Radix Notoginseng were used to perfuse small dose of 5 mg/L, medium dose of 10 mg/L, and high dose of 20 mg/L. 400 μmol/L was given to reperfusion the isolated ischemic hearts, from hemodynamics (LVP, LVEP, CRP, HR), antioxidant free radicals (LPO), cardiac energy metabolism (ATP, ADP, AMP, CAMP) Changes of myocardial injury (LDH, CPK), vascular endothelial activity factors (TXB2, PGF1α), etc. were observed in different doses of the active ingredients of Panax notoginseng compared with the positive drug verapamil and the model group. Results The drug had an effect on the increase of LVP (P<0.05), LVEP, CRP, and HR (P<0.05), and decreased the LPO production (P<0.05); CAMP content increased (P<0.05), ADP, AMP content decreased (P<0.05); LDH, CPK decreased (P<0.05); TXB2 decreased (P<0.05); PGF1α content increased (P<0.05). ); Each effect increases with increasing drug dose. Conclusion Panax notoginseng Rx can increase myocardial contractility, reduce heart rate, increase cardiac output, produce antioxidant free radicals, reduce myocardial energy consumption, reduce myocardial injury, reduce the release of LDH and CPK, and promote the release of endothelial active factors. , protect endothelial cells; and the dose and effect are proportional to each other. It is suggested that the effective component Rx of Panax notoginseng has a protective effect on isolated cardiac ischemia-reperfusion injury.