论文部分内容阅读
目的探讨CIK过继免疫治疗对手术后肺癌患者T细胞及其活化抗原表达的影响。方法采用流式细胞术检测40例肺癌患者外周血T细胞表型CD3、CD4、CD8和及其活化抗原标志CD69、CD25、HLA-DR。结果肺癌患者CD3+、CD4+、CD4/CD8比值明显低于对照组并呈显著性差异,而CD8+细胞高于对照组(P<0.01);肺癌患者CD69、CD3+/HLA-DR+、CD4+/CD25+与对照组比较无显著性差异。经CIK治疗后CD3+、CD4+、CD4/CD8比值都升高,其中CD4+细胞与治疗前比较呈显著性差异(P<0.05),而CD8+细胞与治疗前比较降低(P<0.05);CD69、CD3+/HLA-DR+均升高(P<0.05),CD4+/CD25+在治疗后有增高趋势,但无显著性差异。结论CIK过继免疫治疗对肺癌患者免疫功能的恢复具有促进作用。
Objective To investigate the effect of CIK adoptive immunotherapy on the expression of T lymphocytes and activated antigen in lung cancer patients after operation. Methods Flow cytometry was used to detect T cell phenotype CD3, CD4, CD8 and CD69, CD25, HLA-DR of peripheral blood in 40 patients with lung cancer. Results The ratios of CD3 +, CD4 + and CD4 / CD8 in lung cancer patients were significantly lower than those in control group, but CD8 + cells were higher than those in control group (P <0.01). The levels of CD69, CD3 + / HLA- DR +, CD4 + / CD25 + There was no significant difference between the two groups. After CIK treatment, the ratio of CD3 +, CD4 + and CD4 / CD8 increased, CD4 + cells were significantly different from those before treatment (P <0.05), while CD8 + cells were lower than before treatment / HLA-DR + were increased (P <0.05), and CD4 + / CD25 + increased after treatment, but there was no significant difference. Conclusions CIK adoptive immunotherapy can promote the recovery of immune function in patients with lung cancer.