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目的研究海洋真菌Y26-02发酵液的乙酸乙酯萃取部分,对其中的化学成分进行分离鉴定。方法采用硅胶、Sephadex LH-20柱色谱以及制备型高效液相色谱等手段进行分离纯化,通过理化性质和各种波谱数据对这些化合物进行结构鉴定。结果从海洋真菌Y26-02菌液的乙酸乙酯萃取部分分离得到7个化合物,分别鉴定为灰黄霉素(griseofulvin,1)、表灰黄霉素(epigriseofulvin,2)、异灰黄霉素(isogriseofulvin,3)、脱氯表灰黄霉素(dechloroepigriseofulvin,4)、脱氢灰黄霉素(de-hydrogriseofulvin,5)、4′-丁氧基异灰黄霉素(4′-butoxyisogriseofulvin,6)、2′-丁氧基表灰黄霉素(2′-butoxyepigriseofulvin,7)。结论这7个化合物均为首次从该真菌的代谢产物中分离得到,其中化合物6、7是新天然产物。
OBJECTIVE To study the extraction of the marine fungus Y26-02 fermentation broth by ethyl acetate, and identify its chemical constituents. Methods The compounds were separated and purified by silica gel, Sephadex LH-20 column chromatography and preparative HPLC. The structures of these compounds were identified by physicochemical properties and spectral data. Results Seven compounds were isolated from the ethyl acetate fraction of marine fungus Y26 - 02 and identified as griseofulvin (1), epigriseofulvin (2), griseofulvin (isogriseofulvin, 3), dechloroepigriseofulvin (4), de-hydrogriseofulvin (5), 4’-butoxyisogriseofulvin 6), 2’-butoxyepigriseofulvin (7). Conclusion These seven compounds were isolated from the metabolites of the fungi for the first time, of which compounds 6 and 7 are novel natural products.