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[目的]研究选择性环氧化酶-2(COX-2)抑制剂(塞来昔布)对人外周血单核细胞中RANKL mRNA表达的影响。[方法]人外周血单核细胞应用钛显微粒子刺激后,加入不同浓度的高、低剂量的塞来昔布培养6、12h,以大肠杆菌脂多糖(LPS)为阳性对照组,应用实时定量PCR(RT-qPCR)检测外周血单核细胞中RANKL mR-NA的相对表达量。[结果]人外周血单核细胞受钛显微粒子刺激后,上调RANKL mRNA的表达;低剂量(第3组)和高剂量(第4组)的塞来昔布在给药6 h时,轻度上调RANKL mRNA的表达,而在给药24 h后,显著抑制RANKLmRNA的表达,高剂量组抑制效果更明显。[结论]塞来昔布可抑制钛颗粒刺激后PBMC中RANKLmRNA的表达,从而可能抑制人工关节置换术后由RANKL诱导的破骨细胞增殖活化导致的骨吸收和骨溶解。
[Objective] To investigate the effect of selective cyclooxygenase-2 (COX-2) inhibitor (celecoxib) on RANKL mRNA expression in human peripheral blood mononuclear cells. [Methods] Human peripheral blood mononuclear cells were stimulated with titanium microspheres and cultured with high and low doses of celecoxib at different concentrations for 6 and 12 h. The positive rate of the lipopolysaccharide (LPS) PCR (RT-qPCR) was used to detect the relative expression of RANKL mR-NA in peripheral blood mononuclear cells. [Results] The expression of RANKL mRNA was up-regulated in human peripheral blood mononuclear cells stimulated by titanium microspheres. At 6 h after administration of low dose (group 3) and high dose (group 4) celecoxib, However, the expression of RANKL mRNA was significantly up-regulated after 24 h, but the inhibitory effect was more obvious in high-dose group. [Conclusion] Celecoxib can inhibit the expression of RANKLmRNA in PBMC stimulated by titanium particles, which may inhibit the bone resorption and osteolysis induced by RANKL-induced osteoclast proliferation after artificial joint replacement.