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降钙素基因相关肽(calcitonin gene-relatedpeptide,CGRP)是于1982年在人和哺乳动物神经系统中发现的一种含37个氨基酸残基的神经肽。它具有广泛的生物学效应,对心血管、消化、呼吸、骨胳肌、泌尿、生殖以及免疫等系统均有调节作用。近年来,在免疫细胞中发现了20余种神经内分泌多肽,但迄今为止尚未见CGRP在免疫系统中合成和释放的报道。本文选用大鼠胸腺和肠系膜淋巴结的淋巴细胞,通过放射免疫分析和反相高效液相色谱(HPLC)分析,测定这些淋巴细胞中降钙素基因相关肽免疫活性物质(CGRP-like immunoreactivity,CGRP-LI)的含量,并鉴定其化学性质。
Calcitonin gene-related peptide (CGRP) is a 37 amino acid residue-containing neuropeptide found in human and mammalian nervous system in 1982. It has a wide range of biological effects on cardiovascular, digestive, respiratory, skeletal muscle, urinary, reproductive and immune systems have a regulatory role. In recent years, more than 20 kinds of neuroendocrine peptides have been found in immune cells, but so far no reports of the synthesis and release of CGRP in the immune system have been reported. In this study, lymphocytes of rat thymus and mesenteric lymph nodes were selected for determination of CGRP-like immunoreactivity (CGRP-like immunoreactivity) in these lymphocytes by radioimmunoassay and reverse phase high performance liquid chromatography (HPLC) LI) content, and identify their chemical properties.