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目的:研究半枝莲提取物(SBE)对鼻咽癌CNE-2细胞X-连锁凋亡抑制蛋白(XIAP)表达的影响。方法:体外培养鼻咽癌CNE-2细胞,MTT法观察SBE对CNE-2细胞的抑制作用,免疫细胞化学(SP)法检测SBE对CNE-2细胞XIAP蛋白表达的影响。结果:MTT法结果显示,SBE对CNE-2细胞的抑制率呈浓度依赖性(P<0.01)。与对照组相比,13.44μg.mL-1SBE组XIAP蛋白表达水平明显降低(P<0.01)。结论:SBE能明显抑制鼻咽癌CNE-2细胞增殖。
Objective: To study the effect of Scutellaria barbata extract (SBE) on the expression of X-linked inhibitor of apoptosis protein (CNP) in nasopharyngeal carcinoma CNE-2 cells. Methods: CNE-2 cells were cultured in vitro. The inhibitory effect of SBE on CNE-2 cells was observed by MTT assay. The effect of SBE on XIAP protein expression in CNE-2 cells was detected by immunocytochemistry (SP). Results: MTT assay showed that the inhibitory rate of SBE on CNE-2 cells was concentration-dependent (P <0.01). Compared with the control group, XIAP protein expression in 13.44μg.mL-1SBE group was significantly lower (P <0.01). Conclusion: SBE can significantly inhibit the proliferation of CNE-2 cells.