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目的 观察全脑缺血再灌注后葛根素对神经细胞凋亡相关基因Bcl 2、Bax表达的影响。方法 采用免疫组织化学法、原位末端标记法检测大鼠全脑缺血再灌注不同时间内海马CA1区Bcl 2和Bax蛋白表达水平及凋亡细胞数的变化。结果 ①脑缺血再灌注后 ,海马CA1区Bcl 2蛋白的表达随再灌注时间不同而变化 ,缺血 10min后再灌注 6h达高峰 ;葛根素治疗组Bcl 2蛋白的表达于相应的时间点明显增加 ;②Bax蛋白表达在再灌注 2 4h达高峰 ,葛根素治疗组Bax蛋白表达在相应时间点则下降 ;③神经细胞凋亡数随再灌注时间延长而增加 ,葛根素可减少神经细胞凋亡数。结论 在全脑缺血再灌注后细胞凋亡中 ,Bcl 2、Bax发挥重要作用 ;葛根素通过上调Bcl 2蛋白的表达 ,下调Bax蛋白的表达抑制细胞凋亡发挥神经保护作用
Objective To observe the effect of puerarin on the expression of apoptosis related genes Bcl 2 and Bax after global cerebral ischemia and reperfusion. Methods The expression of Bcl 2 and Bax protein and the number of apoptotic cells in hippocampal CA1 region were detected by immunohistochemical method and in situ end labeling method. Results 1The expression of Bcl-2 protein in hippocampal CA1 region changed with the time of reperfusion, and reached the peak after 10 min ischemia and 6 h after reperfusion. The expression of Bcl 2 protein in puerarin treatment group was obviously at the corresponding time point. Increased; 2Bax protein expression peaked at 24 h after reperfusion, Bax protein expression in puerarin treated group decreased at the corresponding time points; 3 The number of apoptotic nerve cells increased with prolonged reperfusion time, puerarin reduced neuronal apoptosis . Conclusion Bcl 2 and Bax play an important role in apoptosis after global cerebral ischemia-reperfusion. Puerarin can inhibit neuronal apoptosis by up-regulating the expression of Bcl 2 and down-regulating the expression of Bax protein.