结核分枝杆菌的检测,传统的抗酸染色特异性差敏感性低,痰涂片的阳性率在31％左右,胸水的阳性率更低。培养法耗时长,敏感性也不高。自1985年美国Mullis等开展聚合酶链反应(PCR)技术以来,由于其具有快速、敏感和特异等优点广泛地用于临床,特别在结核菌的检测方面取得了一定的成绩。我院应 Mycobacterium tuberculosis detection, the traditional anti-acid stain poor specificity of low sensitivity sputum smear positive rate of about 31%, lower positive rate of pleural effusion. Culture method takes a long time, the sensitivity is not high. Since the United States in 1985, Mullis and other polymerase chain reaction (PCR) technology, because of its fast, sensitive and specific advantages widely used in clinical, especially in the detection of TB has made some achievements. Our hospital should be