论文部分内容阅读
目的建立成体哺乳动物耳蜗螺旋神经节神经元（ｓｐｉｒａｌｇａｎｇｌｉｏｎｎｅｕｒｏｎ，ＳＧＮ）体外培养的细胞模型。方法对出生后７ｄ的Ｗｉｓｔａｒ大鼠的螺旋神经节细胞进行解离与分散培养。用荧光显微镜与倒置相差显微镜观察原代培养ＳＧＮ细胞的活力以及生长与分化过程。应用链霉卵白素过氧化物酶（ＳＰ）方法和抗神经丝蛋白单克隆抗体（ＮＦＰＭｃＡｂ）对ＳＧＮ进行免疫细胞化学染色鉴定。结果幼龄Ｗｉｓｔａｒ大鼠的ＳＧＮ在体外条件下，可良好存活并有正常的表型分化，表现出稳定的神经元可塑性。其细胞数量与形态以及存活周期可满足体外实验的基本要求。结论此方法扩大了内耳组织培养的材料来源，有助于外周听觉系统离体研究的开展。
Objective To establish a cell model of spiral ganglion neuron (SGN) cultured in vitro. Methods Spiral ganglion cells from Wistar rats 7 days after birth were dissociated and dispersed. Fluorescence microscopy and inverted phase contrast microscope were used to observe the viability, growth and differentiation of primary cultured SGN cells. SGN was identified by immunocytochemistry with streptavidin peroxidase (SP) method and anti-neurofilament monoclonal antibody (NFP-McAb). Results SGN in young Wistar rats survived well with normal phenotypic differentiation under in vitro conditions and showed stable neuronal plasticity. The number of cells and morphology as well as the survival cycle to meet the basic requirements of in vitro experiments. Conclusion This method expands the source of material for tissue culture of the inner ear and contributes to the development of the ex vivo auditory system of peripheral auditory system.