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[目的]探讨重组弗林蛋白(Furin)对于类风湿性关节炎(rheumatoid arthritis,RA)滑膜细胞增殖、迁移、侵袭和细胞因子分泌的影响。[方法]从类风湿关节炎患者关节内提取RA滑膜组织后培养原代滑膜成纤维细胞(fibroblast-like synovial cells,FLS);将重组Furin蛋白按照不同浓度(250 ng/ml,500 ng/ml)加入RA滑膜细胞培养基中诱导培养并采用MTT、细胞划痕、Transwell、ELISA等方法检测重组蛋白处理对细胞增殖、迁移、侵袭和炎性因子分泌等生物学特性的影响。[结果]与空白对照组比,加入重组Furin蛋白处理24 h、48 h,对类风湿滑膜细胞增殖活动没有明显影响(P>0.05)。处理24 h后与空白对照组比,迁入划痕伤口内细胞数无显著区别(P>0.05),滑膜细胞穿透基底膜细胞数明显减少(P<0.05),且与剂量浓度呈正相关。培养液上清中IL-1α和IL-17含量升高(P<0.05),而各组间IL-1β和TNF-α含量差异无统计学意义(P>0.05)。[结论]重组Furin蛋白诱导可抑制类风湿关节炎滑膜细胞侵袭能力同时可促进其分泌IL-1α和IL-17。
[Objective] To investigate the effect of Furin on the proliferation, migration, invasion and cytokine secretion of rheumatoid arthritis (RA) synoviocytes. [Methods] Primary synovial cells (FLS) were cultured from rheumatoid arthritis (RA) synovial tissue after RA synovial tissue was removed. Recombinant Furin protein was cultured in different concentrations (250 ng / ml, 500 ng / ml) were added into the synovial cell culture medium of RA for induction culture. The effects of recombinant protein on the biological characteristics of cell proliferation, migration, invasion and secretion of inflammatory cytokines were detected by MTT, cell scratch, Transwell, ELISA and so on. [Result] Compared with the blank control group, the recombinant Furin protein had no significant effect on the proliferation of rheumatoid synovial cells 24 h and 48 h after treatment (P> 0.05). Compared with the blank control group, there was no significant difference between the control group and the blank control group (P> 0.05), and the number of synovial cells penetrating the basement membrane was significantly decreased (P <0.05), and positively correlated with the dose concentration . The content of IL-1α and IL-17 in the culture supernatant increased (P <0.05), while there was no significant difference in the content of IL-1β and TNF-α in each group (P> 0.05). [Conclusion] The recombinant Furin protein can inhibit the invasion of rheumatoid arthritis synovial cells and promote the secretion of IL-1α and IL-17.