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将在线富集技术同二维(2D)毛细管电泳(CE)分离相结合同时提高复杂样品中痕量组分的分离度和检测灵敏度.毛细管区带电泳(CZE)作为第一维,分析物根据淌度不同进行分离,第一维流出组分进入第二维毛细管,根据分配系数不同进行胶束电动毛细管色谱(MEKC)分离.采用阳离子选择性耗尽进样(CSEI)在柱预富集,延长进样时间,增大进样量;同时在二维毛细管接口处采用动态pH联接/胶束扫集在线富集技术不仅避免第一维分离组分在接口处扩散,还可进一步压缩样品区带.同常规电动进样CE分离相比,该在线富集二维分离技术的分离能力远远高于一维CZE或MEKC分离,富集倍数达到(0.5～1.2)×104.该法成功应用于人体尿样中四种药物及对映体的分析测定,浓度检出限为0.1～0.3μg/L.进一步研究了人体尿样中四种药物24h内的药代动力学规律.
The combination of on-line enrichment and 2D (2D) capillary electrophoresis (CE) separation improves the resolution and detection sensitivity of trace components in complex samples.Capillary zone electrophoresis (CZE) The first dimension flowed out into the second dimension capillaries and separated by micellar electrokinetic capillary chromatography (MEKC) according to different partition coefficients.Central selective desiccation injection (CSEI) Prolong the injection time and increase the injection volume. At the same time, the online enrichment technology using dynamic pH coupling / micellar sweep at the two-dimensional capillary interface not only avoids the diffusion of the first dimension of the separation component at the interface, but also further compresses the sample zone With the separation of conventional electrokinetic injection CE compared to the two-dimensional separation enrichment technology online separation efficiency is much higher than the one-dimensional separation of CZE or MEKC enrichment factor (0.5 ~ 1.2) × 104. The successful application of this method The detection limit of four drugs and enantiomers in human urine samples was 0.1 ~ 0.3μg / L. The pharmacokinetics of four drugs in human urine samples were further studied within 24h.