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我组于1980年开始试用液体振荡培养菌丝体,接种培育香菇,获得了正常的子实体。菌种为7402。配料为甘蔗渣100克,锯屑100克,米糠100克,白糖20克,酵母粉(添加剂)2克,水1000毫升。甘蔗渣、锯屑、米糠煮沸后再煮30分钟,过滤取汁,加入白糖和酵母粉再煮沸,pH在6.8～7,分别装入500毫升三角瓶中,每瓶约装300毫升,加玻璃珠12粒,瓶口用八层纱布包扎,高压消毒25分钟后接种,置振荡床上振荡培养。据观察,添加酵母粉的每毫升含香菇菌丝球数,培养4天为94个、5天为104个、6天为56个;而添加硫酸铵的分别为59、81和36个。说明酵母粉含有多种生长因子,对香菇菌丝在液体之中的生长和健壮有促进作用。
In 1980, my group experimented with liquid shaking to culture mycelium, inoculated to cultivate mushrooms and obtained normal fruiting body. The strain is 7402. Ingredients for the bagasse 100 grams, sawdust 100 grams, 100 grams of rice bran, sugar 20 grams, 2 grams of yeast extract (additives), water 1000 ml. Bagasse, sawdust, rice bran boiled and cook for another 30 minutes, filtered juice, add sugar and yeast and boil, pH 6.8 to 7, were loaded 500 ml Erlenmeyer flask, each bottle of about 300 ml, plus glass Beads 12, bottle mouth with eight gauze bandages, high pressure disinfection 25 minutes after vaccination, set the oscillation bed oscillation training. It has been observed that the number of lentinus edodes per milliliter added yeast powder was 94 for 4 days, 104 for 5 days and 56 for 6 days, while 59, 81 and 36 for ammonium sulfate were added. This shows that yeast contains many kinds of growth factors, which can promote the growth and robustness of mushroom mycelium in the liquid.